mcd99In contrast, the anti-mCD99 antibody recognizes the mCD99-Fc used to immunize the rabbits (arrowhead, running at the expected M r of ∼ 75 kD). Display full size. Fure 4Clones expressing mCD99 were harvested either by trypsin or using 10 mM EDTA in Hanks’ balanced salt solution (HBSS). Cells were washed twice in phosphate-bufered sa